DARS (Aspartate--tRNA Ligase, Cytoplasmic, Aspartyl-tRNA Synthetase, AspRS, Cell Proliferation-inducing Gene 40 Protein, PIG40)

Size

£800.00

SKU:: 307004
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Human DARS
Format:: Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Extra Details:: Aminoacyl-tRNA synthetases consist of a family of enzymes that catalyze the specific aminoacylation of cognate tRNA in the initial step of ribosome-dependent protein biosynthesis. DaRS is part of a multisubunit complex of aminoacyl-tRNA synthetases and is involved in the transfer of Asp-tRNA to EF-1 alpha1 (elongation factor alpha 1). The N-terminal of DaRS in vertebrates is a newly evolved structure that contains a putative amphiphilic helix and is dissimilar between different species. The N-terminal extension acts as a switch that, when in its stretched form, reduces the rate of dissociation of Asp-tRNA from DaRS, thereby providing enough time for EF-1 alpha1 to interact with Asp-tRNA. This suggests that the N-terminal of DaRS plays a critical role in its catalytic function. DaRS contains two phosphorylations sites, forms homodimers and localizes to the cytoplasm. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice