303403
ITGB1 (Beta 1 Subunit, CD 29, CD29, CD29 Antigen, Fibrinogen Receptor beta Subunit, Fibronectin Receptor Subunit beta, FNRB, Glycoprotein IIa, GP IIa, GPIIA, Integrin beta 1 (Fibronectin Receptor beta Polypeptide Antigen CD29 Includes MDF2 MSK12), Integrin beta-1, Integrin VLA 4 Subunit beta, Integrin VLA-4 beta Subunit, Integrin, beta 1 (Fibronectin Receptor, beta Polypeptide, Antigen CD29 Includes MDF2, MSK12), ITGB 1, MDF2, MSK12, Very Late Activation Protein beta Polypeptide, Very Late Activation Protein, beta Polypeptide, VLA Beta, VLA-4 Subunit beta, VLA-Beta, VLAB, VLAbeta)
Size
£620.00
- SKU:
- 303403
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human ITGB1 expressed in E. coli. (Position: N527-D728). Species sequence homology: mouse (91%) and rat (88%)
- Format:
- Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
- Extra Details:
- Integrin beta-1, also known as CD29, is a protein that in humans is encoded by the ITGB1 gene. CD29 is an integrin unit associated with very late antigen receptors. It is known to conjoin with alpha-3 subunit to create alpha3beta1 complex that reacts to such molecules as netrin-1 and reelin. This gene contains a beta subunit that appears to be analogous to band-3 of integrin. It is mapped to 10p11.22. It is found that FER mediates crosstalk between CDH2 and CD29. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. The protein encoded by this gene is a beta subunit. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions. Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice