Hsp90alpha (EL52, Epididymis Luminal Secretory Protein 52, Heat Shock 86kD, Heat Shock 90kD Protein 1, alpha, Heat Shock 90kD Protein 1, alpha Like 4, Heat Shock 90kD Protein, alpha-like 4, Heat Shock 90kD Protein 1 alpha, Heat Shock Protein 90kD alpha (Cytosolic) Class A Member 1, Heat Shock Protein 90kD alpha (Cytosolic), Class A Member 2, Heat Shock Protein HSP 90-alpha, HSP 86, HSP86, Hsp89, HSP89A, Hsp90, HSP90A, HSP90AA1, HSP90Alpha, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL3, HSPCAL4, HSPN, LAP 2, LAP2, Lipopolysaccharide-associated Protein 2, LPS-associated Protein 2, Renal Carcinoma Antigen NY REN 38, Renal Carcinoma Antigen NY-REN-38)

Size

£620.00

SKU:: 303368
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human Hsp90 expressed in E. coli. (Position: P2-V365). Species sequence homology: mouse and rat (99%)
Format:: Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Extra Details:: Heat shock protein HSP 90-alpha is a protein that in humans is encoded by the HSP90AA1 gene. The gene, HSP90AA1, encodes the human stress-inducible 90kD heat shock protein alpha (Hsp90A). Complemented by the constitutively expressed paralog Hsp90B which shares over 85% amino acid sequence identity, Hsp90A expression is initiated when a cell experiences proteotoxic stress. Once expressed Hsp90A dimers operate as molecular chaperones that bind and fold other proteins into their functional 3-dimensional structures. This molecular chaperoning ability of Hsp90A is driven by a cycle of structural rearrangements fueled by ATP hydrolysis. Current research on Hsp90A focuses in its role as a drug target due to its interaction with a large number of tumor promoting proteins and its role in cellular stress adaptation. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions. Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Immunocytochemistry: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice