303251
CREB (Active Transcription Factor CREB, cAMP Response Element Binding Protein 1, cAMP Response Element Binding Protein, cAMP Responsive Element Binding Protein 1, cAMP-responsive Element-binding Protein 1, CREB 1, CREB-1, CREB1, Cyclic AMP-responsive Element-binding Protein 1, Transactivator Protein)
Size
£620.00
- SKU:
- 303251
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human CREB expressed in E. coli. (Position: M1-T177). Species sequence homology: mouse (99%) and rat (98%)
- Format:
- Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
- Extra Details:
- CREB1, also known as cAMP responsive element binding protein 1, is a protein that in humans is encoded by the CREB1 gene. It is mapped to 2q33.3. This protein binds the cAMP response element, a DNA nucleotide sequence present in many viral and cellular promoters. The binding of CREB1 stimulates transcription. This protein is a CREB transcription factor that is a member of the leucine zipper family of DNA-binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice