302004

Hypothetical Protein amide [pGlu154] (QRFP-43, QRFP Pyroglutamylated RFamide Peptide 43)

Size

£1,075.00

SKU:: 302004
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Format:: Supplied as a lyophilized powder. Reconstitute with 50ul sterile ddH2O.
Extra Details:: QRFP 43 is a RFamide peptide present in the ventromedial nucleus (VMN) and lateral hypothalamus. It stimulates food intake in mice and its chronic infusion induces hyperphagia, reduced thermogenesis, and obesity. Applications: Suitable for use in Indirect ELISA, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Indirect ELISA: 1:6400 Western Blot: 1:500-1000 Immunohistochemistry(ABC): 1:100-200 Optimal dilutions to be determined by the researcher. Sequence: Glu-Asp-Glu-Gly-Ser-Glu-Ala-Thr-Gly-Phe-Leu-Pro-Ala-Ala-Gly-Glu-Lys-Thr-Ser-Gly-Pro-Leu-Gly- Asn-Leu-Ala-Glu-Glu-Leu-Asn-Gly-Tyr-Ser-Arg-Lys-Lys-Gly-Gly-Phe-Ser-Phe-Arg-Phe-NH2. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.