Endothelin-1 amide (ET-1, Prepro-Endothelin-1) (Not for Export EU)

Size

£1,075.00

SKU:: 301961
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Format:: Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O.
Extra Details:: Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21aa peptides exists in at least three isoforms, ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart and liver. Applications: Suitable for use in Direct ELISA, Immunohistochemistry. Other applications not tested. Recommended Dilution: Direct ELISA: 1:3200 Immunohistochemistry: 1:500 Optimal dilutions to be determined by the researcher. Sequence: Gly-Lys-Asp-Cys-Ser-Lys-Leu-Gly-Lys-Lys-Cys-Ile-Tyr-Gln-Gln-Leu-Val-Arg-NH2 Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.