301790
Vglut1 (Vesicular Glutamate Transporter 1)
Size
£704.00
- SKU:
- 301790
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full Length VGlut1
- Clone:
- McKA1
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile ddH2O.
- Extra Details:
- Glutamate is the major excitatory neurotransmitter in the central nervous system of mammals. Vesicular glutamate transporters (VGluts) are responsible for glutamate transport from the brain into native synaptic vesicles. The level of expression of the three known transporters in the adult brain determines the amount of glutamate that is loaded into vesicles and released. Thus, efficacy of neurotransmission is regulated. 301790 reveals the presence of the 58kD VGlut 1 protein on western Blot of homogenized cerebral cortex cells lysates. It shows very precise reaction with VGlut1 epitope of human mid-frontal gyrus brain tissue in Immunohistochemistry. Applications: Suitable for use in ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 1:500 Immunocytochemistry: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice