STAM (Signal Transducing Adapter Molecule)

Size

£448.00

SKU:: 301764
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: G1G5
Format:: Supplied as a lyophilized powder in PBS, pH 7.4. No preservative added. Reconstitute with 50ul ddH2O.
Extra Details:: The signal transducing adapter molecule 1 (STAM1) and signal transducing adapter molecule 2 (STAM2) are regulators of receptor signalling and trafficking. STAM interacts with the hepatocyte growth factor- regulated tyrosine kinase substrate (Hrs) to form a heterodimeric complex which mediates the endocytic sorting of ubiquitinated membrane proteins. Studies have demonstrated that the complex STAM/Hrs is tyrosine-phosphorylated in response to a variety of growth factors including EGF, HGF and PDGF. STAM1 and STAM2 phosphorylation occurs on tyrosines upon stimulation with cytokines through the Jak3 signaling pathway, which is essential for T-cell development. STAM has an important role in T-cell development and survival in the thymus through the prevention of apoptosis but is not necessary for the proximal signaling of TCR and cytokine receptors. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice