Serotonin (5-Hydroxytryptamine, 5-HT, Neurotransmitter, Enteramine, Thrombocytin, 3-(?-Aminoethyl) -5-Hydroxyindole, 3-(2-aminoethyl)- 1H-indol-5-ol)

Size

£716.00

SKU:: 301751
Purity:: Tissue culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Serotonin prepared from culture medium
Clone:: YC5/45
Format:: Supplied as a lyophilized powder. No preservative added. Reconstitute with 500ul ddH2O. To limit antibody loss or degradation, 1% BSA, 0.02% sodium azide can be added to the suggested first dilution.
Extra Details:: Formaldehyde conjugate of serotonin. Without paraformaldehyde treatment, it cross-reacts in liquid phase with dopamine and tryptamine in hemagglutination tests. The antibody reacts with serotonin in neurons and recognizes serotonergic sites in fixed tissue sections. It does not react with catecholamine-containing neurons in formaldehyde fixed preparations. By immunocytochemistry no cross-reactivity with dopamine and noradrenaline. By red cell agglutination, no cross-reactivity with 5-hydroxyindolacetic acid, GABA, noradrenaline, 5-hydroxytryptophan, carnosine, melatonin Applications: Suitable for use in ChIP, Immunohistochemistry and Agglutination. Other applications not tested. Recommended Dilution: Immunohistochemistry (FFPE): 1:100-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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