Hyphen D12 (SamD12) (Not for Export EU)

Size

£563.00

SKU:: 301676
Application:: WB
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Format:: Supplied as a lyophilized powder. No preservative added. Reconstitute with 100ul ddH2O.
Extra Details:: SamD10 and SamD12 are two recently discovered homolog proteins consisting of a SAM domain. These proteins are also know as Hyphen (HYP) or Aveugle (Ave) and they have been demonstrated to interact with the SAM domain of the Connector enhancer of KSR (CNK) downstream of RAS but a step upstream of RAF. A role for these proteins downstream of EGFR during eye and wing development of Drosophila has also been demonstrated. Heterodimerization of CNK/SAM has been shown to facilitate the recruitment of a KSR/MEK complex to CNK and bridge KSR/MEK to RAF which greatly enhances RAS-mediated RAF activation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.