Huntingtin-Interacting Protein 1-Related (HIP1R)

Size

£550.00

SKU:: 301674
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to human HIP1R at C-terminal.
Format:: Supplied as a lyophilized powder in PBS, pH 7.4, glycerol. Reconstitute with 100ul sterile ddH2O.
Extra Details:: HIP1R (huntingtin interacting protein 1 related) was first cloned based on its sequence similarity with HIP1 (Huntingtin Interacting Protein 1). HIP1 binds to normal huntingtin but this interaction is lost in Huntington’s disease (HD). HIP1 also binds to components of clathrin-coated pits and vesicles and is a marker of clathrin-coated membranes. HIP1R colocalizes with and binds to actin and this interaction may link the endocytic machinery to the actin cytoskeletal dynamic processes. Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:50-1:250 Western Blot: 1:500-1:2500 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.