HHV-6 Variant A IE2

Size

£639.00

SKU:: 301664
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full length IE2
Clone:: P6H8
Format:: Supplied as a lyophilized powder in PBS, pH 7.4. Reconstitute with 100ul sterile ddH2O.
Extra Details:: HHV-6 is a double stranded DNA virus belonging to the betaherpesvirinae subfamily. HHV-6 viruses comprise two distinct species: HHV-6A and HHV-6B. HHV-6 is a T-cell lymphotropic virus with high affinity for CD4 lymphocytes and is capable of infecting virtually all human beings. HHV-6B is responsible for at least 97% of HHV-6 infections in the USA while HHV-6A is frequently associated with patients with neuroinflammatory diseases such as multiple sclerosis. HHV-6A and HHV-6B proteins share more than 92% of their aa composition. However, the immediate-early protein locus A, has less than 70% of homology between the two species. At this locus, IE1 and IE2 proteins are encoded with IE2 being the most divergent between HHV-6A and HHV-6B. IE2 is expressed within few hours post-infection and is a potent transactivator of multiple cellular and viral promoters. Applications: Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:50-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice