Estrogen Sulfotransferase (EST)

Size

£647.00

SKU:: 301644
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Full-length recombinant protein corresponding to bovine EST.
Format:: Supplied as a lyophilized powder in PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile ddH2O.
Extra Details:: Estrogen sulfotransferase (EST) is a cytosolic enzyme that catalyzes specific sulfonation with a high affinity for estrogens. Because EST is involved in estrone and estradiol metabolism, its expression in the male reproductive tract determines where, along the tract, an estrogenic environment predominates. EST may also be involved in sperm physiology during the epididymal transit. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Immunofluorescence: 10ug/ml Immunohistochemistry: 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.