EpCAM-ICD (Epithelial Cell Adhesion Molecule Intracellular Domain)

Size

£659.00

SKU:: 301636
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: 4A7
Format:: Supplied as a lyophilized powder in PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile ddH2O.
Extra Details:: Epithelial cell adhesion molecule (EpCAM) is a 40kD epithelial transmembrane glycoprotein with an ectodomain, one transmembrane domain, and a cytoplasmic domain of 26 residues. EpCAM is frequently overexpressed in human malignancies, normal stem and progenitor cells, and at lower levels in normal epithelia. In vivo and in vitro studies have shown its intracellular domain (ICD) fragment translocates to nucleus after being cleaved from the EpCAM ectodomain and associates with other molecules to bind DNA resulting in cell proliferation and tumor formation. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.