301622
DICER
Size
£659.00
- SKU:
- 301622
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.4. Reconstitute with 50ul ddH2O.
- Extra Details:
- Dicer is an endoribonuclease which has two RNase III domains and one PAZ domain and belongs to the highly conserved family of dsRNA-specific endoribonucleases RNase III family. Dicer plays important roles in RNA processing, post-transcriptional gene expression control and defense against viral infection. Synthesis of proteins can be blocked by RNA in a process known as RNA interference and for this process to happen; three important events have to occur. First, DICER processes dsRNA into smaller fragments known as short interfering RNAs (siRNAs) and microRNAs (miRNAs). Second, a dsRNA binding protein recruits a DICER-siRNA complex to Argonaute2 (Ago2), which is an endonuclease capable of degrading messenger RNA (mRNA). Third, Dicer facilitates the formation of a large multiprotein complex called RISC (RNA-Induced Silencing Complex). RISC can seek out and capture messenger RNA (mRNA) molecules with a base sequence complementary to that of its siRNA or miRNA. This serves to either destroy the genetic message carried by the mRNA outright or else block the subsequent synthesis of a protein. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:3000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice