301620
Cruciform DNA
Size
£698.00
- SKU:
- 301620
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Heteroduplex (cruciform) DNA.
- Clone:
- 2D3
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.4. Reconstitute with 100ul sterile ddH2O.
- Extra Details:
- Inverted repeat sequences are known to form cruciform structures in negatively supercoiled DNA. They are widespread in the genomes of both eukaryotes and prokaryotes, occurring more often than expected among random sequences. In some bacteria, extrusion of cruciform DNA is required for initiation of replication and transcription, though certain inverted repeat sequences are unstable when subcloned into bacteria. Because such structures might impair DNA replication fidelity, the role of inverted repeats in mutagenesis and in human diseases has been widely studied. Applications: Suitable for use in Immunofluorescence, Gel Shift (EMSA) and Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:200 EMSA: 1-10ug Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice