Ahi-1 (itk, Abelson Helper Integration Site 1)

Size

£639.00

SKU:: 301579
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to mouse Ahi-1 at C-terminal.
Clone:: J5
Format:: Supplied as a lyophilized powder. No preservative added. Reconstitute with 100ul sterile ddH2O.
Extra Details:: Abelson helper integration site 1 (Ahi-1) is highly expressed in the most primitive normal hematopoietic cells, in brain neurons and in kidney. Ahi-1 is a unique protein with a SH3 domain, multiple SH3 binding sites and a WD40-repeat domain, which are all known to be important mediators of protein–protein interactions. Defects in Ahi-1 are the cause of Joubert syndrome type 3 (JBTS3). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Immunohistochemistry: 1-5ug/ml Immunoprecipitation: 1-2ug/200-600ug Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.