PCNA (Nuclear Antigen (Proliferating Cells))

Size

£409.00

SKU:: 301548
Purity:: Purified by ammonium sulfate precipitation.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Proliferating Harvey cell line
Clone:: IPO-38
Format:: Supplied as a liquid in PBS, pH 7.2.
Extra Details:: The proliferating cell nuclear antigen (PCNA) becomes detectable in the G1 phase of the cell cycle and reaches a maximum level of expression during mitosis. PCNA is highly conserved across species and is a critical part of the DNA polymerase delta holoenzyme (1,2,3). The level of PCNA expression depends on the proliferative potential of the cell examined. This monoclonal antibody stains proliferating cells in a wide range of normal tissues, as well as in tissues undergoing pathological processes and after mitogenic and allogeneic stimulations. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.