Cyclin E2 (Cyclin E-2) (HRP)

Size

£837.00

SKU:: 301338-HRP
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Residues 2-15 [SRRSSRLQAKQQPQC] of Cyclin E2.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Extra Details:: Cyclin E2 is a novel gene that encodes a protein, which is elevated in cells expressing a papilloma viral oncogene. Cyclin E1 and cyclin E2 can associate with and activate CDK2 whose activity is restricted to the G1-S phase of the cell cycle, and is essential for the G1/S transition. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.