BRCAA1 (Breast Cancer-Associated Antigen 1, BRCAA-1, Retinoblastoma-binding Protein 1-like 1, BRCAA 1) (Biotin)

Size

£837.00

SKU:: 301321-BIOTIN
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Residues aa1046-1055 [SSKKQKRSHK] of 136kD BRCAA1.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: BRCAA1 protein’s epitope, SSKKQKRSHK, has been mapped to the cytoplasm of MCF-7 cells. Immunohistochemistry analysis showed that the antigen epitope exhibited positive expression in 65% of breast cancer specimens. Its expression is associated with characteristics that are associated with prognosis of breast cancer, and its sera antibody level may be helpful for breast cancer diagnosis. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.