299471
Neurogranin (NRGN)
Size
£601.00
- SKU:
- 299471
- Purity:
- Purified by ammonium sulfate precipitation followed by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Synthetic peptide corresponding to C-KIKSGERGRKG, from the internal region of human Neurogranin. Species sequence homology: mouse, canine and bovine
- Format:
- Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
- Extra Details:
- Neurogranin is a calmodulin-binding protein expressed exclusively in the brain, particularly in dendritic spines, and participating in the protein kinase C signaling pathway. Neurogranin is the main postsynaptic protein regulating the availability of calmodulin, binding to it in the absence of calcium. Phosphorylation by protein kinase C lowers its binding ability. Neurogranin is down regulate in Schizophrenia and is not found in the dendrites in patients with Alzheimer disease. Applications: Suitable for use in Peptide ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:8000 Western Blot: 0.1-0.3ug/ml. detects ~16kD band observed in Human Brain (Frontal Cortex) Immunohistochemistry (Paraffin): 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice