ChaT (Choline-O-Acetyltransferase)

Size

£460.00

SKU:: 299341
Application:: IHC-Fr
Purity:: Supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Prokaryotic recombinant protein corresponding to human ChaT at C-terminal.
Clone:: 38B12
Format:: Supplied as a liquid in 15mM sodium azide.
Extra Details:: Murine choline-O-acetyltransferase is a 71,721 dalton protein (640aa) expressed in cholinergic neurons of both the PNS and CNS. In the CNS, ChAT is expressed in motor neurons and pre-ganglionic autonomic neurons of the spinal cord, a subset of neurons in the neostriatum, and in the basal forebrain. In the PNS, ChAT is expressed in a small subpopulation of sympathetic neurons and in all parasympathetic neurons. ChAT is the enzyme responsible for synthesis of acetylcholine from acetyl-coenzyme A and choline. Applications: Suitable for use in Immunohistochemistry. Not recommended for use in Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (Paraffin): 1:25-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.