APP 228 (Amyloid Precursor Protein 228)

Size

£524.00

SKU:: 299301
Application:: IHC-Fr
Purity:: Supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Prokaryotic recombinant fusion protein corresponding to the extracellular protein of APP between the Kunitz protease inhibitor domain and the beta amyloid region.
Clone:: 3G12
Format:: Supplied as a liquid, 15mM sodium azide.
Extra Details:: Alzheimer's disease, the most common cause of dementia in the elderly, exists in both familial and sporadic forms. Genetic studies have identified three genes; beta-amyloid precursor protein (APP), Presenilin 1 and Presenilin 2 which, when mutated, can cause familial forms of Alzheimer's disease. APP and APP-like proteins are transmembrane glycoproteins with a similar modular domain structure. APP-228 has been raised to the extracellular portion of APP between the Kunitz protease inhibitor domain and the beta amyloid region. This region shows the least homology with the APP-like proteins. APP-228 and does not cross-react with APP-like proteins. APP reacts with large pyramidal cells as well as smaller neurons, astrocytes and microglia. APP 228 reacts with late-stage neurofibrillary tangle-bearing neurons, neuritic processes surrounding senile plaques and neuropil threads in gray matter of Alzheimer's disease brain. Unmasking in 1mM EDTA, pH 8.0 in a pressure cooker may be required for up to 5 minutes in order for this APP-228 to work optimally. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Paraffin): 1:25-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice