ALDH1L1 (Aldehyde Dehydrogenase Family 1 Member L1, 10-formyltetrahydrofolate Dehydrogenase, 10-FTHFDH, FTHFD) (Not for Export EU)

Size

£576.00

SKU:: 299291
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein construct of amino acids 1-400 corresponding to human ALDH1L1 expressed in E. coli.
Format:: Supplied as a liquid in 5mM sodium azide.
Extra Details:: ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 expression is tissue-specific and is highly expressed in the liver. In an earlier study, Cahoy et al. applied FACS (Fluorescent-Activated Cell Sorting) to isolate astrocytes from EGFP (Enhanced Green Fluorescent Protein) transgenic-mouse, then created a transcriptome database of the expression levels of 20,000 genes by gene profiling of neurons, astrocytes and oligodedrocytes using Affymetrix GeneChip Arrays. They identified ALDH1L1 as a highly and specifically expressed gene in astrocytes. ALDH1L1 is more widely expressed throughout the brains, while astrocyte marker GFAP shows more predominant expression in white matter. In addition, loss of function or expression of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression, suggesting its role as a biomarker and a target in cancer therapy 299291 stains the expected 100kD band on western blots of crude tissue extracts cleanly and can be used to identify astrocytes in cell culture and sectioned material. Applications: Suitable for use in Immunofluorescence, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence (IC): 1:1000 Western Blot: 1:2000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice