Pro-EGF

Size

£761.00

SKU:: 299220
Purity:: Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to mouse Pro-EGF.
Clone:: 15H126
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
Extra Details:: EGF is the prototypic member of a family of growth factors that also includes amphiregulin, betacellulin, epigen, epiregulin, HB-EGF, neuregulins-1 through -6, and TGF-alpha. These proteins contain EGF-like domains with three intramolecular disulfide bonds between conserved cysteines. EGF family members are synthesized as transmembrane preproproteins with varying numbers of EGF-like domains. The extracellular region of mouse pro-EGF contains eight LDL R class B repeats and nine EGF-like domains. Mature EGF is derived from the juxtamembrane EGF-like domain. EGF binds ErbB1 and induces the formation of homodimers or heterodimers containing ErbB2. Pro-EGF is most highly expressed in the submaxillary gland and kidney. In the kidney, the 190kD preproprotein is cleaved by membrane-associated serine proteases, liberating the extracellular region which is subsequently processed into smaller fragments including the 6kD mature EGF. The various cleavage products produced in the kidney also are present in urine. In the submaxillary gland, however, nearly all EGF is processed intracellularly and stored in secretory vesicles. The soluble precursor binds ErbB1 and induces cellular proliferation, although it is significantly less potent than mature EGF. In human thyroid carcinoma cells, a splice variant of pro-EGF with a deletion in the cytoplasmic domain induces increased proliferative activity relative to wild-type pro-EGF. Within the extracellular region, mouse pro-EGF shares 79% amino acid sequence identity with rat pro-EGF and 67%-69% with human, canine, feline, and porcine pro-EGF. Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-2000 Immunohistochemistry(Frozen): 1:50-200 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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