Metadherin

Size

£761.00

SKU:: 299191
Application:: WB
Purity:: Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to mouse Metadherin.
Clone:: 15H97
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
Extra Details:: Metadherin (MTDH; also Lyric and Astrocyte-elevated gene-1/AEG1) is a unique molecule originally discovered to be upregulated in astrocytes following HIV infection. Although its predicted MW is 64kD, it runs anomalously at ~80kD in SDS-Page. It is widely expressed, and appears to be a component of the ER, nucleolus and inner nuclear membrane. Metadherin is reported to promote Akt/PI3 kinase activity, and suppress apoptosis-associated FOXO3A transcription. Mouse Metadherin is a type III (i.e.- a type I with no signal sequence) transmembrane protein 579 amino acids (aa) in length. It contains a luminal N-terminus (aa1-48) with a 510aa cytoplasmic C-terminus. There are no identifiable structural motifs, although two poly-Lys segments and two utilized Ser phosphorylation sites exist. Multiple bands at 80kD, 75kD, 50-55kD and 37kD are seen for Metadherin on SDS-Page. They may reflect the presence of at least two potential isoform variants that show 1) a deletion of aa422-457, and 2) an Ile substitution for aa190-579. Over aa167-297, mouse Metadherin shares 98% and 97% aa sequence identity with rat and human Metadherin, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice