299164
Integrin beta 4 (CD104)
Size
£761.00
- SKU:
- 299164
- Purity:
- Purified by Protein G affinity chromatography from culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to mouse Integrin beta 4.
- Clone:
- 15H75
- Format:
- Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Integrin beta 4, also known as CD104, is a 200kD type I transmembrane (TM) glycoprotein that associates only with Integrin alpha 6/CD29. Integrin alpha 6 beta 4 is predominantly expressed by epithelial cells, binds laminins, and is essential for formation of hemidesmosomes and connection of the dermis to the epidermis. Mouse integrin beta 4 contains a 680 amino acid (aa) extracellular domain (ECD) with a metal-binding site and four cysteine-rich repeats, a 22aa TM segment and an unusually long 1073aa cytoplasmic tail that organizes hemidesmosome components. Isoforms with shortened cytoplasmic tails have been described. Mouse and human beta 4 ECD show 88% aa identity. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-2000 Immunohistochemistry(Frozen): 1:100-200 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice