299120
Coagulation Factor VII
Size
£761.00
- SKU:
- 299120
- Purity:
- Purified by Protein G affinity chromatography from culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to mouse Coagulation Factor VII.
- Clone:
- 15H31
- Format:
- Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Coagulation Factors VII and VIIa refer to the pro and active forms of the same protease, respectively. Factor VII is synthesized in the liver and circulates in the plasma where it binds to tissue factor (TF), an integral membrane protein found in a variety of cell types. Upon binding of TF, Factor VII is rapidly converted into VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis. The cleavage and activation of Coagulation Factors VII, IX and X by VIIa:TF is phospholipid-dependent whereas the cleavage of small peptide substrates is not. The predominant splicing variant of Factor VII in normal liver corresponds to the 444 amino acid precursor. After a signal peptide (aa1-38), the mature chain can be further processed into the light chain (aa39-190) and the heavy chain (aa191-444). Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-2000 Immunohistochemistry(Paraffin): 1:50-100 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice