299090
AIRE (Autoimmune Regulator)
Size
£761.00
- SKU:
- 299090
- Purity:
- Purified by Protein G affinity chromatography from culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to mouse AIRE.
- Clone:
- 15H1
- Format:
- Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
- Extra Details:
- AutoImmune REgulator (AIRE) is an approximately 60kD nuclear and cytosolic protein that is required for the development of T cell tolerance. It regulates the expression of self-antigens by thymic epithelial cells, and mutations in AIRE are causative of the autoimmune disorder, APECED. AIRE regulates gene transcription through interactions with DNA, histone H3, and the nuclear matrix. It contains one HSD domain (aa1-105), a nuclear localization sequence (aa113-133), one SAND domain (aa181-280), and two PHD zinc finger domains (aa299-340 and aa434-475). Alternate splicing of human AIRE generates isoforms that lack the HSR and SAND domains and/or the second PHD domain. Within aa476-552, human AIRE shares 65% and 63% aa sequence identity with mouse and rat AIRE, respectively. Applications: Suitable for use in Flow Cytometry, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice