BRPF3 TR-FRET, BioAssay™ Kit (KIAA1286)

Size

£1,567.00

SKU:: 298383
Storage Conditions:: -20°C/-70°C
Supplier:: United States Biological
Extra Details:: Kit comes in a convenient format with purified BRPF3 protein, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions. The BRPF3 TR-FRET BioAssay™ Kit is designed to measure the inhibition of BRPF3 binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRPF3, substrate, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader. Applications: Suitable for use in screening small molecular inhibitors for drug discovery and HTS applications. Other applications not tested. Kit Components: White, Nonbinding Corning, low volume, microtiter plate, 1x plate GST-BRPF3, 1x10ug BET Bromodomain Ligand, 1x50ul Non-acetylated Ligand 1, 1x15ul Tb donor, 1x20ul Dye-labeled acceptor, 1x20ul 3x BRD TR-FRET Assay Buffer 1, 1x4ml Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.