Heparan Sulfate Proteoglycan (HS-PG)

Size

£351.00

SKU:: 298187
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified human small basement membrane heparan sulfate proteoglycan (HS-PG).
Clone:: 2E2/B5
Format:: Supplied as a lyophilized powder in 50mM TRIS, pH 7.4. Reconstitute with sterile ddH2O.
Extra Details:: Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that at least two other basement membrane HS-PG exist: Agrin, originally discovered as an important component of the neuromuscular junction, and a novel small HS-PG which was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200kD (aorta) and 30-160kD (kidney) and a core protein size of 24kD or 22kD, respectively, was localized by immunohistochemistry to the basement membrane. Amino acid sequence analysis of tryptic peptides indicates that this small HS-PG is clearly distinct from perlecan and agrin. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: <1ug/ml Western Blot: 1ug/ml Immunohistochemistry: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice