Epidermal Transglutaminase, Recombinant, Human (TGM3, TGe, Protein-Glutamine gamma-Glutamyltransferase E, EC=2.3.2.13, Transglutaminase E, Transglutaminase-3)

Size

£341.00

SKU:: 298170
Molecular Weight:: 77
Purity:: ~95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in 50mM HCl, pH 7.5, 1mM EDTA, 1mM HABA, 1mM PMSF. Reconstitute with sterile ddH2O.
Extra Details:: Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath. Source: Recombinant protein corresponding to human Epidermal Transglutaminase, expressed serum-free in a human cell line. Molecular Weight: ~77kD Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: coating antigen at 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice