S100A9 (S100 Calcium Binding Protein A9, Myeloid-related Protein 14, MRP 14, Calgranulin B)

Size

£991.00

SKU:: 298097
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Complete mouse S100A9 protein
Clone:: MU14-2A5
Format:: Supplied as a liquid in PBS, 0.1% BSA.
Extra Details:: The monoclonal antibody S100A9 recognizes S100A9. The calcium-binding, migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. MRP14 is co-expressed with human MRP8, a homologue protein in myeloid cells, and plays an essential role in calcium dependent functions during inflammation. This role includes the activation of Mac-1, the beta2 integrin which is involved in neutrophil adhesion to endothelial cells. MRP14 is distinguished from other S100 member proteins by its long C-terminal region. The expression of MRP-8 (S100A8) and MRP-14 (S100A9) is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. In inflammatory conditions small venueles stain with both anti-S100A8 and S100A9. The staining of the two subunits is always coincident. The S100A8/A9 complex has antibacterial, antifungal and immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot (Reduced): 1:50. The band size is 13-15kD. Immunohistochemistry (Paraffin): 1:50 Immunohistochemistry (Frozen): 1:50 Optimal dilutions to be determined by the researcher. Positive Control: Mouse bone marrow cell extracts Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice