MAP44

Size

£1,068.00

SKU:: 298081
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: MAp44 specific peptide
Clone:: 2D5B6
Format:: Supplied as a liquid in PBS.
Extra Details:: The monoclonal antibody MAp-44 reacts specifically with human MAp-44, a protein of ~44kD, which is encoded by an alternatively spliced mRNA of the MASP-1 gene. Up to now 5 MASP proteins have been identified (MASP-1, MASP-2, MASP-3, MAp19 and MAp44) which are encoded by 2 genes. The MASP-2 gene gives rise to MASP2 and MAp19 due to alternative splicing, whereas the MASP-1 gene encodes a primary transcript which can be spliced to three different mRNAs coding for MASP-1, MASP-3 and MAp44. On the protein level MASP-1 and MASP-2 are shown to be responsible for complement activation through proteolytic cleavage of C2 and C4, whereas MASP-3 has been suggested to inhibit the generation of the C3 convertase. So far the role of MAp44 remains elusive. However, recent research have shown that patients suffering from colorectal cancer have increased levels of MAp44 upon surgery as compared to Map44 levels in normal sera. The monoclonal antibody MAp-44 is suitable for immunohistochemistry, western blotting as well as use in immunoassays. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:50 Western Blot: 1:50 Immunohistochemistry(Paraffin): 1:50 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice