Ficolin-2 (Ficolin-2/P35, L-Ficolin, Ficolin L, EBP-37, Hucolin)

Size

£1,138.00

SKU:: 298070
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: GN5
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Extra Details:: The monoclonal antibody Ficolin-2 recognizes human Ficolin-2, also known as L-ficolin. Ficolins are a family of pattern recognition molecules containing both a collagen-like domain and a fibrinogen-like domain that are capable of activating the complement system, through association with MASPs, on the surface of microorganisms. In human, three Ficolins are known: Ficolin-1 (M-ficolin), Ficolin-2 (L-ficolin), and Ficolin-3 (H-ficolin). Ficolin-1, is mainly expressed by monocytes, granulocytes and lung cells and is present locally in areas of inflammation. Ficolin-2 (also L-ficolin, ficolin L, EBP-37 or hucolin) is produced primarily by the liver. The association between Ficolin-2 and MASPs/sMAP is Ca2+-dependent. MASPs in Ficolin-2- MASP complexes have proteolytic activities for C4, C2 and C3. Ficolin-2 also shows opsonic activity. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: non-reducing conditions, the band size is ~35kD. Immunohistochemistry: Frozen. Tissue sections were fixed in acetone and pretreated with peroxidase for 5 min. in the dark at RT to quench endogenous peroxidases. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice