Endothelial Cell Antigen (RECA)

Size

£745.00

SKU:: 298065
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: RECA-1
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Extra Details:: Endothelial cells (EC) line the interior of all blood vessels and are the key players in the angiogenesis cascade. EC are the first cells and barrier that vehicles or medicines encounter after systemic delivery. Furthermore, they have a signaling function to the cells of the immune system to indicate the status of the surrounding tissue. RECA-1is reactive with the rat MHC-haplotype; Lewis (TR-1), BN (RT-1) and OA (RT-1). RECA-1 antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of the RECA-1 monoclonal antibody was seen with other cell types e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens. Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 Immunohistochemistry(Frozen): 1:50 Immunohistochemistry(Paraffin): 1:50 Optimal dilutions to be determined by the researcher. Positive Control: Glomerular endothelial cells, RHEC cell line Negative Control: Vascular smooth muscle cells, fibroblasts Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice