EMAPII (Endothelial Cell and Monocyte Activating Proinflammatory Cytokine, Endothelial-monocyte-activating Polypeptide II)

Size

£876.00

SKU:: 298064
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to full-length mouse EMAP II
Clone:: M7/1
Format:: Supplied as a liquid in PBS, 0.1% BSA.
Extra Details:: EMAPII is a proinflammatory cytokine and chemoattractant of macrophages and polymorphonuclear lymphocytes. The protein was initially identified as a product of murine methylcholanthrene A-induced fibrosarcoma cells. EMAP II is synthesized as a 34kD precursor molecule (proEMAP), it can run anomalously in SDSpage at 43kD, and is enzymatically cleaved to produce a biologically active 22kD mature polypeptide. It modulates a range of properties of endothelial cells, monocytes, and neutrophils in vitro, and induces an acute inflammatory reaction and tumor regression in vivo. Later it was found to be anti-angiogenic and capable of induction of apoptosis in proliferation and hypoxic endothelial cells in vitro in tumor vasculature in vivo. EMAP II is released from cells, by a yet unknown mechanism, as pro- and mature EMAP II proteins in response to various forms of cellular stresses, including glucose starvation and hypoxia. Endothelial cells are the main target for EMAPII and explain the anti-angiogenic property. The anti-tumor effect is expected to a large extent to be mediated through this characteristic. EMAPII is also associated with brain diseases and injury. EMAPII expression is related with leukocytes- infiltrated inflamed tissue and subsequent apoptosis. In solid tumors there is also an immunosuppressive role identified. EMAPII inducing tumor cells induce apoptosis of lymphocytes. EMPAII is considered a marker for microglial cells and macrophages in brain diseases. The distribution of EMAPII and its precursor in biopsies might be applicable as additive diagnostic tool. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:50 Western Blot: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice