CFHR1-2 (Complement Factor H Related Protein, CFHR)

Size

£991.00

SKU:: 298059
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Mouse IgG1
Clone:: JHD-7.10.1
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Extra Details:: The monoclonal antibody CFHR1-2 reacts with human complement factor H-related protein 1 and 2 (CFHR1/2). In humans the complement factor H protein family consists of complement factor H, the factor H-like protein 1 and five factor H-related proteins termed 1 to 5. All members of this group are expressed primarily in the liver and subsequently secreted into plasma. The secreted forms of these proteins are composed exclusively of repetitive protein domains termed short consensus repeats (SCR). The CFHR1 protein is composed of five SCR domains and circulates in plasma as two differentially glycosylated isoforms (37kD and 43kD). CFHR-2 is composed of four SCRs and is present in human plasma in a non-glycosylated form (24kD) and a glycosylated form of 29kD. So far the biological functions of both CFHR-1 and CFHR-2 proteins are poorly understood, however recent studies have shown that CFHR1 is a complement regulator that blocks C5 convertase activity as well as assembly and membrane insertion of the terminal components. Moreover, genetic variations in the CFHR1 gene are associated with several diseases such as atypical hemolytic uremic syndrome and age-related macular degeneration. Due to a high degree of amino acid sequence homology the monoclonal antibody is cross-reactive with both CFHR1 and 2. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:50 Western Blot: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice