CD46 (Membrane Cofactor Protein)

Size

£876.00

SKU:: 298050
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Second and third SCR domains of MCP fused to IgG-Fc; MCP23-Ig
Clone:: MM1
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Extra Details:: The monoclonal antibody CD46 recognizes CD46, also known as membrane cofactor protein. An immunohistological survey of rat tissues revealed that CD46 is expressed only in the testis and epididymis of adult rats more specifically, only by spermatozoa and late spermatozoal precursors. In contrast, in humans, CD46 is expressed on every cell and tissue, with the exception of erythrocytes. CD46 serves to down-regulate the activation of complement on host tissue. It functions as a cofactor by binding to C3b and C4b. Binding is permitted by factor I, a serine protease of plasma, to degrade C3b and C4b and serves to protect the host cell against autologous attack. In human, it also serves as a receptor for measles virus. Two isoforms of CD46 predominate and arise by alternative splicing of a single CD46 gene. The isoforms differ in the length. CD46 cDNA encodes a signal sequence followed by four complement control protein domains (also called short consensus repeats (SCR)). Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:50 Western Blot: 1:50 Immunohistochemistry(Frozen): 1:50 Optimal dilutions to be determined by the researcher. Positive Control: Western blotting: rat testis lysate, non-reducing conditions, band size 38kD Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice