CD200R3 (CD200R3, CD200RLb, OX-2 Receptor 3)

Size

£1,068.00

SKU:: 298047
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Mouse primary basophils
Clone:: Ba91
Format:: Supplied as a liquid in PBS, 0.1% BSA.
Extra Details:: The monoclonal antibody CD200R3 recognizes mouse CD200 Receptor-like 3 (CD200R3), a protein of ~38kD or ~74kD (reduced or non-reduced condition, respectively). CD200R3 presumably exists as a disulfide-linked dimer on the cell surface. The OX-2, or CD200 receptor (CD200R), belongs to the immunoglobulin superfamily. CD200R contains two Ig-like extracellular domains and mediates inhibitory signals in myeloid cells. Mice deficient for the ligand CD200 develop enhanced experimental allergic encephalomyelitis and collagen-induced arthritis. Unlike CD200R, the CD200R-like receptors functions as activating receptors. They contain short cytoplasmic tails and a lysine residue in the transmembrane region and are likely to signal via adaptor proteins such as DAP12, DAP10, FcRgamma, or CD3zeta. CD200R3 is expressed preferentially on cells of the myeloid lineage, including mast cells and basophils. Both cell types are involved in the host defense system against pathogens and in the development of allergic disorders. They function as independent essential initiators of allergic reactions. Basophils are the least common leukocytes in the peripheral blood accounting ~0.5% of all leukocytes. In vivo depletion of basophils by using monoclonal antibody proved the essential role of basophils in the development and maintenance of IgE-mediated chronic allergic inflammation. The activating CD200 receptors, like CD200R3, play an important role in IgE-independent mast cell and basophil activation. Monoclonal antibody CD200R3 activates basophils ex vivo and elicits systemic anaphylaxis when administered in vivo. Cross-linking of CD200R3 on MC/9 cells by monoclonal antibody CD200R3 induces degranulation. Monoclonal antibody CD200R3 is also useful for depletion of basophils. Applications: Suitable for use in Flow Cytometry, ELISA, Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice