298040
Beta-Defensin 2 (HBD-2)
Size
£991.00
- SKU:
- 298040
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- CHPVFCPRRYKQIGTCGL
- Format:
- Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
- Extra Details:
- The polyclonal antibody recognizes human beta-defensin 2 (hBD-2). hBD-2 is a cystein-rich cationic 41 amino acid antimicrobial peptide of 4-5kD. Human BD-2 is produced by epithelial cells upon stimulus by lipopolysaccharides and proinflammatory cytokines TNFalpha and IL1beta. Contact of keratinocytes with gram-negative bacteria results in rapid induction of hBD-2 protein. hBD-2 has been described as a dynamic component of the local epithelial defense system of the skin, intestinal and respiratory tract, where it functions by protecting surfaces from infection. Its local expression has been associated with skin lesions like psoriasis as well as infected lung epithelia of patients with cystic fibrosis. Furthermore, in inflammatory bowel disease (IBD), expression of hBD-2 is increased in patients with IBD compared to healthy persons. The NF-kB pathway has been recognized as a key component in the induction of hBD-2 expression, however, other studies have observed induction mediated by the mitogen-activated protein kinase (MAPK) pathways. Thus, increased expression of hBD-2 in epithelial cells is associated with the proinflammatory response. This is supported by the finding that the anti-inflammatory cytokines IL-10 and IL-13 downregulate the synthesis of hBD-2 in atopic dermatitis. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:50 Western Blot: 1:50. The band size is 25kD for recombinant protein with tag and 5kD for the native protein. Immunohistochemistry(Paraffin): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice