Nitrocefin

Size

£480.00

SKU:: 258056
Molecular Weight:: 516.5
Storage Conditions:: -20°C
Supplier:: United States Biological
CAS Number:: 41906-86-9
Extra Details:: Nitrocefin is a chromogenic β-lactamase substrate that undergoes colour change from yellow to red as the amide bond in the β-Lactam ring is hydrolyzed by β-lactamase. Nitrocefin undergoes colour changes induced by lactamases produced by Gram-positive and Gram-negative bacteria. Several studies have utilized the colour changing properties of Nitrocefin for the detection of β-lactamase activity from bacterial cell extracts by isoelectric focusing and spectroscopy. Nitrocefin has also been used in studies involving β-lactamase resistant antibiotics. Synonyms: (6R,7R)-3-[(1E)-2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid; [6R-[3(E),6α,7β]]-3-[2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid CAS No: 41906-86-9 Molecular Formula: C₂₁H₁₆N₄O₈S₂ Molecular Weight: 516.50 Purity: ≥98% Appearance: Orange to red solid Solubility: DMF: 20 mg/ml; DMSO: 20 mg/ml Melting Point: 103-113°C (dec.) (lit) Storage and Stability: May be stored at RT for short-term only. Long-term storage is recommended at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.