Coelenterazine, native (Luciferin, Preluciferin)

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£479.00

SKU:: 257643
Molecular Weight:: 423.46
Purity:: ≥95%
Storage Conditions:: -20°C
Supplier:: United States Biological
CAS Number:: 55779-48-1
Format:: Brownish/yellow to yellow/orange crystalline powder
Extra Details:: Coelenterazine is a luciferin, a light-emitting biomolecule that serves as a substrate for luciferases or as a constituent of photoproteins, including aequorin.1 The oxidation of coelenterazine to coelenteramide is accompanied by emission of blue light (emission maximum, 460-470 nm). Luciferase-mediated oxidation of coelenterazine or a derivative is used as an energy donor, typically to a form of green or yellow fluorescent protein, in bioluminescent resonance energy transfer studies.2,3 Alternatively, the calcium-mediated release of coelenterazine from aequorin, followed by non-enzymatic oxidation of this compound, results in bioluminescence. As light emission depends on both calcium and cellular redox status, this reaction is used to non-fluorescently detect changes in calcium level and redox status.4,5 Synonyms: 3,2-Dihydro-2-(p-hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazolo[1,2-a]pyrazin-3-one; CLZN; Preluciferin; Luciferin; 6-(4-hydroxyphenyl)-2-[(4-hydroxyphenyl)methyl]-8-(phenylmethyl)-imidazo[1,2-a]pyrazin-3(7H)-one CAS No: 55779-48-1 Molecular Formula: C₂₆H₂₁N₃O₃ Molecular Weight: 423.46 Appearance: Brownish/yellow to yellow/orange crystalline powder Purity: ≥95% Solubility (0.0125mg/ml in methanol: Orange to yellow to brown liquid Molar Extinction Coefficient (0.0125mg/ml in methanol, lambda max1): >7800.0 L/(mol cm) UV (0.0125mg/ml in methanol, lambda max1): 431-435nm UV (0.0125mg/ml in methanol, lambda max2): 261-265nm Storage and Stability: Store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Hygroscopic and light sensitive. Store in amber vial under inert atmosphere. Note: It is not recommended to store dissolved coelenterazine at -20°C or -70°C. Coelenterazine is a high energy dioxetanone ring structure that will spontaneously decompose even at low temperatures.

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