VSIG1 (V-set and Ig Domain-containing Protein 1, GPA34, Cell Surface A33 antigen, V-set and Immunoglobulin Domain Containing 1)

Size

£761.00

SKU:: 257188
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Val23-Glu234 from extracellular domain of mouse VSIG1, expressed in NS0 cells. Species Sequence Homology: rat; 94%, human; 81%
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS.
Extra Details:: VSIG1 (V-set and Ig domain-containing protein 1; also Glycoprotein 34 and Cell surface antigen 33) is a 55-70kD member of the JAM family of Ig-Superfamily proteins. It shows expression on gastric epithelium and testicular germ cells and likely participates in cell adhesion. Mouse VSIG1 precursor is 407 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 211aa extracellular domain (ECD) (aa23-234) and a 152aa cytoplasmic region. The ECD contains one V-type (aa23-134) and one C2-type Ig-like domain (aa145-229). Over aa23-234, mouse VSIG1 is 94% and 81% aa identical to rat and human VSIG1, respectively. There is one potential alternate start site at Met108. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice