254322
8-Oxoguanine (Ascites Free)
Size
£991.00
- SKU:
- 254322
- Purity:
- Purified by Ion Exchange chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- 8-oxoguanine adsorbed onto alumina
- Clone:
- 483.15
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide. Ascites free.
- Extra Details:
- 8-Oxoguanine is a mutagenic oxidative damage product of guanine. Oxidatively damaged base 8-oxoguanine is generated in the DNA of all living organisms due to the presence of reactive oxygen species in cells. DNA damage threatens the health of the genetic information stored in every DNA molecule; however enzymes exist in cells to protect against the mutagenic effect of this lesion. 8-oxoguanine is one of the most abundant and well-characterized DNA lesions generated by oxidative stress. It has been estimated that ~180 guanines are oxidized to 8-oxoG per mammalian cell per day. 8-oxoG is a miscoding lesion that can cause G:C to T:A or T:A to G:C transversion mutations. This lesion accumulates in DNA with age and it has been loosly linked to several cancers and diseases, such as Alzheimer's and Parkinson's. Applications: Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:50 in HeLa cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice