Spi2A (Spi2A, Serine Protease Inhibitor 2A, Serine Protease InhibitorA3G, Serpin 2A, Serpin A3G, Serpina3g)

Size

£731.00

SKU:: 254297
Application:: WB
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Hamster
Immunogen:: Synthetic peptide corresponding to Mouse Spi2A aa406-426 (C terminal). Sequence: NPERSTNFPNGEGASSQRC
Clone:: MoFo29.2
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Spi2A is a serine and cysteine protease inhibitor. It can inhibit lysosomal papain-like proteases including the cathepsins B G H K L and V. It is ineffective against elastase granzyme A granzyme B or caspases 3 8 or 9. Inhibition of cytoplasmic cathepsin B following release from the lysosome may protect cells from apoptosis. This may facilitate the survival of progenitor T-cells and the subsequent development of long term memory CD8 T-cells. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.