254151
LAP2 alpha (OIP5, 5730547N13Rik, Cancer/testis antigen 86, CT 86, CT86, LAP2alpha interactor 25, LINT 25, LINT25, MIS18beta, OIP 5, Opa Interacting Protein 5) (Not for Export EU)
Size
£607.00
- SKU:
- 254151
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein fragment corresponding to aa188-693 of Human LAP 2 alpha, fused to His-Tag.
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Extra Details:
- Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A- type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins lamin B receptor emerin and MANI three isoforms of lamina-associated polypeptide 1 (LAP 1) and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha which are identical in their N-terminal 187aa constant region but differ in their C terminal. While LAP 2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth. LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunocytochemistry and ChIP. Other applications not tested. Recommended Dilution: Western Blot: 1:2500. Detects a band of ~83kD. Immunocytochemistry/Immunofluorescence: 1:250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice