HIV1 Vif (SOR Protein, Virion infectivity Factor)

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£731.00

SKU:: 254094
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 Vif protein (clade B, HXB-3 isolate)
Clone:: 319
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: HIV1 Vif (viral infectivity factor), supports productive viral replication in non-permissive cells, such as peripheral blood lymphocytes. It functions by inactivating a cellular anti-viral factor named APOBEC3G. Vif binds viral RNA and affects the stability of viral nucleoprotein core. May play a role in viral morphology. Interacts with host ABCE1, which seems to be involved in lentiviruses capsid formation and displays RNase L inhibitor activity. This interaction may play a role in protecting viral RNA from damage during viral assembly Applications: Suitable for use in Immunofluorescence, Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Predicted molecular weight: 23kD Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0-Ag.14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice