254048
GAD65 (65kD Glutamic Acid Decarboxylase, DCE 2, DCE2, GAD 2, GAD 65, GAD-2, GAD-65, GAD2, Glutamate Decarboxylase 2 (Pancreatic islets and brain 65kD), Glutamate Decarboxylase 2, Glutamate Decarboxylase 65, Glutamate Decarboxylase65kD isoform, Glutamic Acid Decarboxylase 2, Glutamic Acid Decarboxylase 65)
Size
£731.00
- SKU:
- 254048
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide: GSGFWSFGSEDGSGDSEN conjugated to KLH, corresponding to aa4-22 of human GAD65.
- Clone:
- 144
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Extra Details:
- GAD65 catalyzes the formation of gamma-aminobutyric acid from L-glutamic acid. It is an autoantigen in type I diabetes and is transiently activated in response to the demand for extra GABA in neurotransmission. It cycles between an active holo form and an inactive apo form. The gene on chromosome 10p12 is a candidate for human obesity. The cDNA encodes a Mr 65,000 polypeptide, with 585aa residues. The pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. Applications: Suitable for use in RIA, Flow Cytometry, Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml. Predicted molecular weight: 65kD Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0-Ag.14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice