253983
CD45RO (PTPRC, T200) (HRP)
Size
£774.00
- SKU:
- 253983
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Cultured T cells from an IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus.
- Clone:
- UCH-L1
- Format:
- Supplied as a liquid in PBS, 0.01% Thiomersal. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Receptor-type tyrosine-protein phosphatase C (PTPRC), a 180kD transmembrane protein, which is also known as CD45. CD45 is present on all leukocytes. CD45R and CD45RO are two of multiple PTPRC isoforms generated by alternative splicing. CD45R is found on B lymphocyte and T lymphocyte subsets. CD45RO is present on 50% of T cells, most granulocytes and monocytes and most mature T cell tumors. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice